Tuesday, March 19, 2019
Analysis of the Absorption of Green Light Versus Red Light Absorption i
abbreviation of the Absorption of Green Light Versus Red Light Absorption in Spinach LeavesThe goal of the experiment was to determine if green weak had little ability to imbibe than red flicker in spinach leaves. This was through by separating the photosynthetic pigments (chlorophyll a, chlorophyll b, carotene and xanthophylls) from one another use paper chromatography. The separated pigments were then analyzed for their ingress spectrum using a spectrographometer. When the data was graphed it clearly showed the high rate of red elation absorption over green light. These results along with previous research indicate the enormousness of red light in photosynthesis and the minor role green light plays. The majority of life on Earth depends on photosynthesis for food and oxygen. Photosynthesis is the transmutation of carbon dioxide and water into carbohydrates and oxygen using the suns light qualification (Campbell, 1996). This process consists of two parts the light re actions and the Calvin cycle (Campbell, 1996). During the light reactions is when the suns energy is converted into ATP and NADPH, which is chemical energy (Campbell, 1996). This process occurs in the chloroplasts of plants cell. Within the chloroplasts are multiple photosynthetic pigments that absorb light from the sun (Campbell, 1996). Photosynthetic pigments work by absorbing antithetical wavelengths of light and reflecting others. These pigments are divided into two categories primitive (chlorophyll) and accessory (carotenoids) pigments. chlorophyl is then divided into three forms a, b, and c (Campbell, 1996). Chlorophyll a is the primary pigment used during photosynthesis (Campbell, 1996). This pigment is the only one that can immediately participate in light reactions (Campbell, 1996). Chlorophyll a absorbs the wavelengths of 600 to 700nm (red and orange) along with four hundred to 500nm (blue and violet) and reflects green wavelengths (Lewis, 2004). Chlorophyll b has only a slight departure in its structure that causes it to have a different absorption spectra (Campbell, 2004). The carotenoid convoluted with spinach leaf photosynthesis absorbs the wavelengths of 460 to 550nm (Lewis, 2004). The pigments are carotene and its oxidized derivative xanthophylls (Nishio, 2000). A wavelength is determined by measuring from the hint of one wave to the crest of the next wave. All the wavelengths possible are... ...2520 0.06 0.049 0.01 0.005 0.09540 0.06 0.06 0.01 0 0.088560 0.08 0.065 0.01 0 0.09580 0.125 0.076 0 0 0.111600 0.15 0.091 0 0.005 0.122620 0.21 0.09 0 0.005 0.148640 0.24 0.3 0 0.005 0.195660 0.99 0.18 0.01 0.01 0.495680 0.18 0.03 0 0.01 0.09700 0.03 0.01 0 0 0.028720 0.01 0.01 0 0 0.02Figure 2 The absorabancy spectrum show s how absorbent the photosynthetic pigments are at different wavelengths of light. Note Green light is between 500 to 570 nm and red light is between 630 to 720 nm. ReferencesCampbell, N.A., Biology, New York The Benjamin/Cummings create Company, Inc., 1996, 182-200.Karohl, D., Principals of Biology I Laboratory, Lorain, Lorain County Community College, 2003, 65-71.Lewis, R., Life, Boston McGraw-Hill , 2004, 97-114.Nishio, J.N., Why are higher plants green? Evolution of the higher plant photosynthetic pigment complement, Plant, kiosk and Environment, 2000, 23, 539-5
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